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. 2008 Apr 17;27(10):1502–1512. doi: 10.1038/emboj.2008.81

Figure 2.

Figure 2

Resection of a DSB is faster in dot1Δ, rad9Y798Q and rad9Δ cells. (A) WT (JKM179), dot1Δ (YFL399), rad9Y798Q (YFL504) and rad9Δ (YFL419) cells, carrying a unique HO-cut site at the MAT locus and expressing the HO endonuclease under the inducible GAL1 promoter, were grown in presence of lactate and arrested with nocodazole. HO was induced with galactose 2%. Genomic DNA, extracted from samples collected at the indicated times, was digested with SspI and separated on alkaline denaturating gels. Resection was monitored by Southern blotting using a ribo-probe specific for the 3′strand. (B) Scheme of the MAT locus. The figure shows the positions of the HO-cut site, and of the probe (asterisk) used for the experiments shown in (A). The black vertical bars indicate the SspI sites. The products of the digestion of differently resected molecules are shown above the scheme.