Figure 4.

Affinity-purified anti-peropsin antibodies recognize a single protein with an apparent molecular mass of approximately 38 kDa. Fifteen micrograms of total protein from mouse brain or mouse eye from which the lens was removed were loaded on each lane of a 10% polyacrylamide/SDS gel and either stained with Coomassie blue (Left) or immunoblotted using affinity-purified rabbit antibody prepared against a synthetic 20 amino acid peptide corresponding to the carboxyl-terminus of mouse peropsin (Right). The molecular masses of protein size standards, indicated at the left, are, from top to bottom, 192, 127, 73, 43, 32, and 17 kDa.