Figure 5.

Histochemical localization of peropsin in the adult mouse eye. Albino (CD1) mice were used in all experiments so that RPE melanin would not obscure the signal. (A) Immunoperoxidase and (B) immunofluorescent staining with affinity-purified anti-peropsin antibodies localizes peropsin to the apical face of the RPE. (B) Nuclei are visualized with 4′,6-diamidino-2-phenylindole. (C) Immunogold staining with anti-peropsin antibodies of a 1 μm section of unicryl-embedded tissue localizes peropsin to the apical microvilli of the RPE. Gold particles were silver-enhanced and the section was counterstained with methylene blue. Photoreceptor outer segments are visible in the lower half of the micrograph. (D) Confocal image of anti-peropsin immunostaining of the RPE. Peropsin is localized to the dense network of microvilli that protrude from the apical face of the RPE; occasional larger structures (double arrowhead) protrude from the RPE and contain peropsin immunoreactivity. Single arrowhead, nucleus of RPE cell. CH, choroid; OS, outer segments; ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer; AV, apical microvilli.