Figure 1.

The influence of EGF on the expression of BAD and phosphorylated BAD in cultured human trophoblasts. A, A representative immunoblot of four isoforms of BAD in trophoblasts cultured in standard conditions with vehicle (control) or 100 ng/ml EGF, the EGF receptor tyrosine kinase inhibitor AG1478 (10 μm), or both. B, Densitometric analysis of the six primary cultures examined. Vehicle control vs. EGF exposure for phosphorylated BADser112 (P < 0.01) and phosphorylated BADser155 (P < 0.05) was significant (ANOVA with Bonferroni correction).