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The influence of hypoxia and EGF on the expression of BAD and phosphorylated BAD. The upper panel (A) is an immunoblot of the isoforms of BAD and actin in trophoblasts cultured in a FiO2 = 20% with vehicle (control) or FiO2 less than 1% (hypoxia) in the presence or absence of 100 ng/ml EGF. The lower panel (B) is the densitometric analysis of isoforms (n = 6) normalized to actin. *, P < 0.05 vs. control (ANOVA with Bonferroni correction).
