Figure 4.

Comparison of adiponectin complex distribution in human vs. mouse serum samples. The data from the human serum samples in Fig. 3 was compared with data obtained from male and female mice. A, A representative complex distribution of adiponectin from female mouse serum (n = 5) compared with female human serum samples (n = 4). B, A representative complex distribution of adiponectin from male mouse serum (n = 5) compared with male human serum samples (n = 4). Serum samples from female mice (20 μl) and male mice (25 μl) were injected onto the gel filtration column equilibrated in HEPES/Ca²⁺ buffer. The relative intensity of each fraction is plotted against the retention volume for mouse (□) and human (•). Quantification of the complex distribution of adiponectin was shown for females (B) and males (D). The percentage of each complex was calculated by dividing the area under the curve for each peak by the total area under the curve for the entire spectrum.