Figure 6.

Complex distribution of adiponectin in the supernatant and cellular extract. Day 8 in vitro differentiated 3T3-L1 adipocytes were incubated with 3 ml serum-free DMEM, and the supernatant was collected after 4 h. The medium was loaded on a gel filtration column, and the complex distribution of adiponectin was determined by Western blot using antimouse adiponectin antibodies. The same cells were treated in lysis buffer with Triton X-100. Cellular extracts were separated by gel filtration using the HEPES/Ca²⁺ buffer plus Triton X-100, and the complex distribution was analyzed in a similar fashion.