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. 2008 Jan 10;149(5):2369–2382. doi: 10.1210/en.2007-0922

Figure 1.

Figure 1

Correlation between serum TNF-α levels, the inhibition of hepatic insulin (Ins) signaling, and the extent of hemorrhage after trauma alone or trauma and hemorrhage. Rats were subjected to T or trauma and hemorrhage. In the trauma and hemorrhage group, rats were bled to a mean arterial pressure of 60–65, 45–50, or 35–40 mm Hg within 10 min and then maintained for 90 min. A, Serum TNF-α levels. Data are presented as the mean + sem of samples from four to eight rats in each group. B, Saline (−) or 5 U insulin (+) was injected into the portal vein, and 1 min later the liver was removed, and protein extracts were prepared, resolved by SDS-PAGE, and subjected to Western blot analysis using specific anti-PY612-IRS1, anti-IRS1, anti-PS473-Akt, and anti-Akt antibodies. Representative Western blots are presented. The original blots were cut and rearranged for the sake of clarity. C and D, Autoradiographs were quantified by scanning densitometry. Data are presented as the mean ± sem of samples from four to eight rats in each group. In this and all the following figures: * or #, P < 0.05; ** or ##, P < 0.01; and *** or ###, P < 0.001. BP, Blood pressure.