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. 2008 Jan 10;149(5):2369–2382. doi: 10.1210/en.2007-0922

Figure 3.

Figure 3

Phosphorylation and activity of JNK1/2 increased in rat liver after trauma and hemorrhage. At 0′ (after T, but before any further treatment), 90′ (without or with hemorrhage), or 210′ (without hemorrhage or with hemorrhage and 60 min recovery after 60 min resuscitation), the liver was removed. A, Protein extracts were subjected to Western blot analysis with specific anti-P-JNK1/2 and anti-JNK1/2 antibodies. Representative Western blots are presented. C, Protein extracts were subjected to in vitro JNK kinase activity assay using c-Jun as the substrate. Representative Western blots are presented. HC indicates the heavy chain of immunoprecipitated anti-JNK antibody. B and D, Data are presented as the mean ± sem of samples from four to eight rats in each group.