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. 2008 Jan 10;149(5):2369–2382. doi: 10.1210/en.2007-0922

Figure 5.

Figure 5

Exogenous TNF-α infusion induced JNK phosphorylation and IRS-1 S307 phosphorylation in rat liver. TNF-α at a dose of 125 μg/kg body weight (in 1 ml saline containing 0.2% BSA) or 1 ml saline containing 0.2% BSA was infused via the portal vein over a period of 30 min at a constant infusion rate. A and B, Blood was collected at 30 min of TNF-α or saline infusion, or at 60 min after a 30-min infusion. Serum TNF-α and IL-6 levels were examined. C, Sixty minutes after 30 min of TNF-α or saline infusion, either saline or insulin was injected into the portal vein, and 1 min later the liver was removed. Liver protein was subjected to Western blot analysis with specific anti-P-JNK, anti-JNK, anti-PS307-IRS-1, and anti-IRS-1 antibodies. Representative Western blots are presented. D and E, Data are presented as the mean ± sem of samples from three rats in each group.