Figure 6.

Insulin (Ins)-induced hepatic tyrosine phosphorylation of IRS-1 and phosphorylation of Akt were inhibited by exogenous TNF-α infusion. At the same time points and treatment regimens described in Fig. 5, the liver was removed, and protein extracts were subjected to Western blot analysis by specific anti-PY612-IRS-1, anti-IRS-1, anti-PS473-Akt, and anti-Akt antibodies. A, Representative Western blots are presented. B and C, Data are presented as the mean ± sem of samples from three rats in each group. $, P < 0.05. $$, P < 0.01. $$$, P < 0.001. NS, Not significant.