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. Author manuscript; available in PMC: 2008 Apr 22.
Published in final edited form as: Immunobiology. 2007 Dec 3;212(9-10):759–769. doi: 10.1016/j.imbio.2007.09.019

Fig. 2.

Fig. 2

Hmp lessens NO-mediated inhibition of SPI2 transcription. (A) Expression of SPI2 was estimated as β-galactosidase activity of a spiC::lacZ transcriptional fusion expressed in hmpA-proficient or –deficient Salmonella. Bacterial samples were collected 2 h after culture in 8 μM MgCl2 N salts medium in the presence of increasing concentrations of the NO donor spermine NONOate. (B) The NO2--producing capacity of control and IFNγ-primed macrophages was measured by the Griess reaction 12 h after challenge. (C) Intracellular hmpA expression was quantified following expression of the hmpA::lacZ transcriptional fusion 20 h after infection.