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. Author manuscript; available in PMC: 2008 Oct 15.
Published in final edited form as: Arch Biochem Biophys. 2007 Jul 10;466(2):164–171. doi: 10.1016/j.abb.2007.06.027

Table 1.

Efficacy of the ABTS•+ reduction, loss of ABTS•+ during the fast phase of the reaction and the initial rate of the reduction of ABTS•+ by DOX, p-QH2, Trolox C and GSH in aerated buffer pH 7.0.

Reductor Δ[ABTS•+]/Δ[reductor] Δ[ABTS•+]/5 s (% of initial level)d Initial rate, µM/mine
  1.8 ± 0.1a 48.4 ± 1.3 22.0 ± 2.0
DOX 3.7 ± 0.4b    
       
p-QH2 1.9 ± 0.1a 75.6 ± 1.5 23.4 ± 0.3
       
Trolox C 2.1 ± 0.1a 84.6 ± 1.8 25.6 ± 0.3
       
GSH 1.8 ± 0.1c 50.8 ± 1.7 15.7 ± 0.2
a

For the fast process (first 5 s of the reaction).

b

After 6 min.

c

After 1 min.

d

Amount of ABTS•+ lost upon the addition of 9.9 µM p-QH2, Trolox C, GSH, or 6.9 µM DOX measured during the first 5 s after the reductor addition.

e

Initial rates were determined based on ΔA415 over the first 5 s of the reaction and are expressed per 1 µM of a reductor.