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Active site of Y295F trichodiene synthase complexed with Mg2+3-PPi. (a) Simulated annealing omit maps of PPi and Mg2+ ions are shown in cyan (9.8Å) and maroon (7.5Å), respectively. (b) Superposition of the active sites of PPi-bound wild-type (purple) and Y295F (magenta) trichodiene synthases. Note that the metal ion coordination and hydrogen bond interactions are similar in the wild-type (purple) and Y295F trichodiene synthases (black and gray).
