. Author manuscript; available in PMC: 2009 Jan 15.

Published in final edited form as: Arch Biochem Biophys. 2007 Oct 30;469(2):184–194. doi: 10.1016/j.abb.2007.10.015

Table II.

Kinetic Parameters of Mutant Trichodiene Synthases

Trichodiene synthase	k_cat (s⁻¹)	K_M (μM)	k_cat/K_M (M⁻¹s⁻¹) (x 10⁶)
Wild-type^a	0.138 ± 0.004	0.078 ± 0.0056	1.77
N225D^b	0.005 ± 0.0003	0.485 ± 0.016	0.01
S229T^b	0.015 ± 0.004	6.0 ± 0.8	0.0025
N225D/S229T^b	inactive	-	-
Y295F^b	0.111 ± 0.005	0.069 ± 0.005	1.59

From reference 26.

Standard deviations for k_cat and K_m are the calculated statistical errors for the nonlinear least-squares regression fit of the data to the Michaelis-Menten expression, with R values typically >0.98.