Table 3.
Microsatellites isolated from BAC clones and mapped on the linkage map of L. calcarifer
| Orginal order | Linkage group | Locus | GenBank accession no | Motif | Forward primer (5'-3') | Reverse primer (5'-3') | PCR Ta (°C) | Product Length (bp) | Location in the library (384plate-96plate-well)* |
| 1 | LG1 | LcaB003 | EU072356 | (GT)14 | CCTCATACTTGCATCAACATAATA | ATCAAAACCGGCTTCATCT | 55 | 113 | 128-P2-A3 |
| 2 | LG1 | LcaB030 | EU072367 | (CA)27 | TTCTCCCCGTGCCCCTTTGTA | AGCCCACTCCCCTGAGATGAGC | 55 | 158 | 128-P2-B8 |
| 3 | LG2 | LcaB128 | EU072400 | (GT)16 | AGTCGGCCTGTGCAATAAGAT | CAGCAGTTTGGGAATAATGACATA | 55 | 262 | 128-P1-E4 |
| 4 | LG3 | LcaB002 | EU072355 | (GT)10 | TTGGCTGTATTCCTCCTGTCTTGT | TTGGCTCTTTACGCTCAATACTCA | 55 | 182 | 128-P2-A4 |
| 5 | LG4 | LcaB012 | EU072359 | (CA)9 | GTGGGGTGTCCTGGCTCCTC | TCCCATCTCCTCCTGCTGTTTCT | 55 | 329 | 128-P2-B11 |
| 6 | LG4 | LcaB014 | EU072361 | (GT)16 | GCAGACCCGCTTTTTATTCAT | GTCCCCTCTGCTCCAGTGTT | 55 | 181 | 128-P2-C12 |
| 7 | LG4 | LcaB052 | EU072376 | (CA)10 | ATCATGACCCACAAGAGGAGAG | TCAGGGATAGAGACTTGTGAATGA | 55 | 146 | 128-P2-A5 |
| 8 | LG4 | LcaB053 | EU072377 | (GT)18 | GAGGCCCCGATGAGAAAACCTG | TGATGTCGGCGGAGGAGTGC | 55 | 319 | 128-P2-H11 |
| 9 | LG5 | LcaB034 | EU072369 | (CA)8 | TTTGCCTGAATAAAACCCTACACT | AAGCCCTTTGCACAGTATTATTTC | 55 | 171 | 128-P2-C8 |
| 10 | LG5 | LcaB084 | EU072391 | (GT)14 | GAGCGCTCGGCTGTTTCATC | CAGCCAATCTGTTTACCAGCACAC | 55 | 248 | 128-P2-C4 |
| 11 | LG5 | LcaB086 | EU072392 | (CA)12 | CAGATGATCTTTGACGAACTGAAA | TTCTTGGTGAAAAATGACAACAAA | 55 | 157 | 128-P3-C5 |
| 12 | LG5 | LcaB130 | EU072401 | (CA)26 | GGGGGAAAGGAAAAACTGATG | TGTAATGGTAAGATTTTGGGTGTG | 55 | 215 | 128-P2-F6 |
| 13 | LG5 | LcaB177 | EU072409 | (GA)13 | TTTAATTTTAGCCCCGTGATT | GTGTGCCAGTGGGTTTCTC | 55 | 214 | 128-P3-C1 |
| 14 | LG5 | LcaB180 | EU072410 | (TC)13 | AGTCTACACCGATTACACCAGTCT | ACTCTAACCGCACCAGAAAAG | 55 | 243 | 128-P2-C7 |
| 15 | LG5 | LcaB229 | EU072417 | (GT)14 | ACATCGCGTTCTCCTCTGAT | CCAGGGTGTGGTAGTCCTCTC | 55 | 140 | 128-P3-C8 |
| 16 | LG6 | LcaB065 | EU072384 | (GT)13 | GCATTGTTGGCAAAGTTGAGTAT | TCTTACAGTGGGCATCTGACCT | 55 | 148 | 128-P3-G1 |
| 17 | LG6 | LcaB188 | EU072411 | (GA)17 | TGATTTGGCTTTTAGGTGAAACA | TGACAAAAGAATGCCTTGCTCT | 55 | 211 | 128-P3-D7 |
| 18 | LG7 | LcaB010 | EU072358 | (CA)9 | TCCTCCTGGGCTGTTGTATCTTAT | ATGGGGTGGACCTCATTTTCA | 55 | 155 | 128-P1-G10 |
| 19 | LG7 | LcaB072 | EU072385 | (GT)10 | CAACGTGGGTGAATCTGTGT | TTGGCAGCAAATAATTCAGAGTAT | 55 | 217 | 128-P1-A11 |
| 20 | LG7 | LcaB114 | EU072395 | (AC)8 | TGTGCCCATGTTTACTAGATACCA | GTGTGCACGCTGCATTTGT | 55 | 172 | 128-P2-F9 |
| 21 | LG7 | LcaB135 | EU072402 | (TC)18 | CATCCCAGGTTTTCATACCATT | ACTGCGGTTATTAATCCACAAAG | 55 | 123 | 128-P3-C4 |
| 22 | LG7 | LcaB151 | EU072404 | (TC)11 | TTGTGCGCTTCTGTTTGTTTTTCT | GTAGGGCTATGCTGTTGGCTTTCT | 55 | 311 | 128-P2-D2 |
| 23 | LG8 | LcaB025 | EU072366 | (GT)13 | AGGGGGCAAAGGGGTCACG | GAGCCGGCAGTTGCACATCTG | 55 | 160 | 128-P2-B3 |
| 24 | LG8 | LcaB083 | EU072390 | (GT)12 | CGCTGGCATGGCTCTAGTAGTGAT | AGCGGGCTAAAAGCTGCTGTG | 55 | 366 | 128-P1-H5 |
| 25 | LG8 | LcaB214 | EU072413 | (GA)12 | AGCGGGAGGCTGAGAAGTAA | ACCCCTGCCTCTTGTTCATC | 55 | 239 | 128-P1-H4 |
| 26 | LG9 | LcaB024 | EU072365 | (GT)10 | AGAAGGGAAAAAGAGATGGGATGT | CAGGGCCGTTTTATTGCTGTAG | 55 | 162 | 128-P3-B2 |
| 27 | LG9 | LcaB045 | EU072373 | (GT)26 | ACAGGGAACGAATGGGGACAA | AAATTGGCACGCTCATTCAAGAAC | 55 | 149 | 128-P2-D4 |
| 28 | LG9 | LcaB155 | EU072405 | (GA)24 | TGTGGCCTTTGTGTAAGTGAGAA | TCATTCCCGCAAACAACACA | 55 | 197 | 128-P3-G11 |
| 29 | LG10 | LcaB160 | EU072406 | (GT)13 | CTTCATCCAGCCCAGTGACAG | GAATGGCCAGCTAAAACATCAAC | 55 | 307 | 128-P3-A1 |
| 30 | LG10 | LcaB201 | EU072412 | (TC)16 | ATTGCACCAGTCCCGAATGAG | GCAGCGTGCTTGTGGAAAAA | 55 | 210 | 128-P2-D1 |
| 31 | LG11 | LcaB112 | EU072394 | (GT)7 | TACCTGCCTTGTTTTTGTCCTTA | AAGCCTCCATACACAGCTACATTA | 55 | 113 | 128-P1-D6 |
| 32 | LG12 | LcaB041 | EU072371 | (AC)8 | AGGTATGTTTTTGGGGCTTTTAGT | CCCCCTACCCCTGTTTTACATA | 55 | 250 | 128-P1-B5 |
| 33 | LG12 | LcaB058 | EU072381 | (AC)15 | AAACCAAATGCTTACACAGTTACC | TTGAGAGCTATTGGGATTACACAT | 55 | 160 | 128-P1-A2 |
| 34 | LG13 | LcaB059 | EU072382 | (AC)18 | CCTAGCCAAGTGCAACAGTGTG | AGCTGGGAAACAGGCTGAGAC | 55 | 186 | 128-P3-A12 |
| 35 | LG14 | LcaB055 | EU072379 | (AC)12 | AGTTGCGGTCTTGTCCAAATGG | ACTGGCAGAGTCAAGCAAAGTGTG | 55 | 325 | 128-P3-A3 |
| 36 | LG14 | LcaB075 | EU072386 | (GT)12 | TGTCGCACACCGCTGCTTTACTAT | CTTGCTCTCACCCTCTCCCTCTTT | 55 | 131 | 128-P2-G12 |
| 37 | LG14 | LcaB076 | EU072387 | (AC)17 | CGAAAACGTCGATCCAACTAAA | ACAGTCAGTGCGTGAAGTGTATG | 55 | 135 | 128-P3-A2 |
| 38 | LG14 | LcaB127 | EU072399 | (AC)11 | AGTTGCAGGGCATGCTGTGAAAC | TCGGCATCAAGCGTGGAAGAG | 50 | 159 | 128-P2-D6 |
| 39 | LG15 | LcaB174 | EU072408 | (GT)8(GA)15 | CAGCATTAAAAAGATGAGAAAAGT | ATTCCCCCATCTTTGTTACAGTT | 55 | 242 | 128-P2-D7 |
| 40 | LG16 | LcaB013 | EU072360 | (AC)15 | AGGCCAAGGCTGCTCTGTGTC | CAACCTGGGATGAGGCACTAAAAG | 55 | 127 | 128-P2-B12 |
| 41 | LG16 | LcaB054 | EU072378 | (AC)8 | TGCAGGAGATAAGACGCTGTG | GAGATCGGCAACCTGACAAA | 55 | 298 | 128-P3-F4 |
| 42 | LG16 | LcaB062 | EU072383 | (AC)15 | ATGAGGGGTGAACAGTTGTCCT | TCTCCTCGTCCTTTTCGTTACC | 55 | 218 | 128-P3-F8 |
| 43 | LG16 | LcaB078 | EU072388 | (GT)13 | GTTACCATGCCAACAACCAA | TAGCCTGCTATAGATCCCACTG | 55 | 81 | 128-P3-A4 |
| 44 | LG16 | LcaB228 | EU072416 | (AC)21 | GAATAGGCCTACCTGGTGAGAGG | TCCCTGCTTAGCTGCCATTATC | 55 | 237 | 128-P2-B12 |
| 45 | LG17 | LcaB023 | EU072364 | (GT)13 | GCAGCGAGATGAACAGTGATTATT | ACATGATCCTCGCCACCATC | 55 | 326 | 128-P2-G5 |
| 46 | LG17 | LcaB048 | EU072374 | (GT)20 | TGGAGCTTTATTTGAGTGTGAC | CCCCCTATGTATTCAGTATTCTG | 55 | 180 | 128-P3-C4 |
| 47 | LG17 | LcaB051 | EU072375 | (GT)19 | TACCCAAAGTAAACCAGCAGCACA | CAACTAGCAGGTTTGCACAACACA | 55 | 104 | 128-P3-H11 |
| 48 | LG17 | LcaB121 | EU072397 | (AC)16 | CTTTTTGTGCCCCAGATGACG | GGAGCAGAGTGGAGCTTTCAGAA | 55 | 238 | 128-P1-D9 |
| 49 | LG18 | LcaB019 | EU072363 | (GT)11 | TTGAGTCCCCTGTGCTATGTAACA | CACCGCCTCCACAATTAGTGTC | 55 | 199 | 128-P1-F10 |
| 50 | LG18 | LcaB081 | EU072389 | (GT)7(GCA) (GT)3 | TGAGGACAGCCACCCCACTTTT | GAGCCGCTATCTCATTCCCACATC | 55 | 126 | 128-P2-F10 |
| 51 | LG18 | LcaB221 | EU072415 | (TC)9 | AGGGGAGTGCTGCCTCAGTG | TTCCCAACAGATAATGATGCTCAA | 55 | 117 | 128-P3-A8 |
| 52 | LG19 | LcaB005 | EU072357 | (AC)22 | AGGCGGTGCTGGGGCAGAT | TTACCGCAGCCTGGCTAGAGGTCT | 55 | 300 | 128-P3-H8 |
| 53 | LG19 | LcaB033 | EU072368 | (AC)15 | ATCCACCTTGAGGTTTCTTTATCA | AACCAAGCCACTCCTATCATCTT | 55 | 190 | 128-P1-D5 |
| 54 | LG20 | LcaB219 | EU072414 | (GA)25 | AGTTGGCTCTTAAAGCATTTGAAT | TTCCCACACCGTTAGGTTTATCTG | 55 | 155 | 128-P1-H12 |
| 55 | LG21 | LcaB106 | EU072393 | (GT)7 | CTGGCTGCATGGAGAAAGAAGT | TTGGGTTTTGAGCTCACTGACA | 55 | 311 | 128-P2-F7 |
| 56 | LG21 | LcaB116 | EU072396 | (GT)20 | CATGGCCTTTCTGGGAAGTTATTG | CAGACGGAGCCACAAGCAAAAC | 55 | 226 | 128-P3-D6 |
| 57 | LG21 | LcaB169 | EU072407 | (AC)6(GA)20 | CACAAACCAGGCGATCACATATCG | GTAAGCCCGCAGAAATCGACTTCA | 55 | 218 | 128-P3-E9 |
| 58 | LG23 | LcaB015 | EU072362 | (GT)11 | GAGCGCTCTCCCCTGGTTTC | TGCAGCCGAGCACGACTG | 55 | 221 | 128-P1-G9 |
| 59 | LG23 | LcaB038 | EU072370 | (GT)19 | TGTGCGCACTCACATACATTAG | TGAAAAATAGATGGTAAGCCTCTC | 55 | 216 | 128-P1-A3 |
| 60 | LG23 | LcaB056 | EU072380 | (AC)11 | ATGCCGTTTCCTGCTGCTGTC | TGATGCTGTTTCTGGCTGGTGTA | 55 | 141 | 128-P2-E02 |
| 61 | LG23 | LcaB150 | EU072403 | (GA)11 | TCTAGCGCTCGTCCTCTCCTG | AGGCCTCCTCGTTCTCTGCT | 55 | 178 | 128-P2-A11 |
| 62 | LG24 | LcaB125 | EU072398 | (GT)12 | AAGCACAAGATACGCCTTCCTT | GTGCCCTGGGCCTCTACAT | 55 | 153 | 128-P2-C11 |
| 63 | Unlinked | LcaB044 | EU072372 | (GT)15 | CAGGACGTTTGAATACTTGTGT | TTAAAAGGTGGTGGTATTAGTCAT | 55 | 160 | 128-P2-A8 |
Ta: annealing temperature for PCR. * 384plate-96plate-well: name of the 384-well plate, name of 96-well plate and well position in the 96-well plate.