Table 1.

Details of the immunohistochemical stainings

Antibody	Species	Company and institutes	Dilution	Incubation	Antigen retrieval	Detection
HIF-1α	Mouse	BD Pharmingen	1/500	30′ RT	WB TRS 97°C 45′	CSA
Akt	Rabbit	Cell Signaling	1/50	o/n 4°C	WB Citr 97°C 10′	ABC
mTOR	Rabbit	Cell Signaling	1/50	o/n 4°C	WB Citr 97°C 10′	ABC
VHL	Mouse	BD Pharmingen	1/100	60′ RT	WB Citr 97°C 20′	Powervision
CA IX	Mouse	Lab Harris	1/50	30′ RT	none	Envision+
Glut-1	Rabbit	DAKO	1/400	60′ RT	WB Citr 97°C 20′	ABC
SDF-1	Mouse	R&D systems	1/400	o/n 4°C	WB Citr 97°C 10′	ABC
Hydroxyprobe-1	Mouse	Chemicon International	1/200	40′RT	0.01% pronase in PBS 40°C for 40′	ABC

BD Transduction laboratories, Mab monoclonal antibody, PAb polyclonal antibody, RT room temperature, WB water bath, TRS target retrieval solution (DAKO), Citr citrate buffer 10 mM pH 6.0, CSA catalyzed signal amplification system (DAKO), ABC avidin–biotinylated peroxidase complex, Powervision from immunologic R&D systems