Figure 2.

Detection of substitutions with hMC-derived mass spectra in the heterozygous and homozygous state. Mass spectra of the T reverse reaction of a wild type (top), heterozygous (middle), and homozygous (bottom) C253T mutation in exon 7 of Parkin. The sequence in the left part of the figure indicates the variation and the masses of the indicative fragments (underlined). The substitution from G to A in the reverse reaction results in a mass shift of −16 da. The peaks correspond to the mutant (mut) and wild-type (wt) fragment, respectively.