Table 4.

Discrepancies between porA Results: Capillary-Based Sequencing and MRM Resequencing

MRM ID	Method	porA variant	DNA sequence
70	Cap*	4–0	CATGTTGTTGTGAATAAC AAGGTTGCTACTCACGTTCCGGCT
70	MRM†	4–2‡§	CATGTTGTTGTGAATAACAAGGTTGCTACTCACGTTCCGGCTAAGGTTGCTACTCACGTTCCGGCT
59	Cap	10–4	CATTTTGTTCAGAATAAGCAAAATAAGCAAAAT CAGCCGCCTACTCTCGTTCCG
59	MRM	10–10	CATTTTGTTCAGAATAAGCAAAATAAGCAAAATAAGCAAAATCAGCCGCCTACTCTCGTTCCG
12	Cap	23–2	CATTGGAATACTGTGTATAACACTAACGGTACTACTACTACTACT TTCGTTCCGGCTGTT
12	MRM	23–3	CATTGGAATACTGTGTATAACACTAACGGTACTACTACTACTACTACTTTCGTTCCGGCTGTT

^*Where Cap indicates capillary-based sequencing.

^†Where MRM indicates MRM microarray resequencing.

^‡Bold type indicates sequence differences between porA variants.

^§Repeated sequences are underlined.