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. Author manuscript; available in PMC: 2008 Apr 23.
Published in final edited form as: Chem Res Toxicol. 2006 Jul;19(7):894–898. doi: 10.1021/tx060076u

Figure 2.

Figure 2

hAS3MT mRNA levels in parental and clonal HepG2 cells. (a) PCR products generated by touch-down PCR from hAS3MT and β-actin cDNA. Negative control (NC) represents a PCR reaction, in which cDNA was replaced with water. Positive control (PC) represents a PCR reaction in which cDNA was replaced with pRSET/hAS3MT expression vector. (b) hAS3MT mRNA levels quantified by real-time PCR. Mean and SD values for three independent measurements are shown. *The mean value is significantly different (p < 0.001) from that in parental HepG2 cells.