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. 1997 Sep 2;94(18):9926–9931. doi: 10.1073/pnas.94.18.9926

Figure 2.

Figure 2

Immunofluorescence microscopic labeling with live, or with fixed and permeabilized, DAMI cells, using as the primary antibody reagent either the anti-PS-2 antibodies of Fig. 1B, or anti-tubulin antibodies in single indirect labeling experiments, with fluorescein-tagged secondary antibodies. (a) Live cells, transfected with the pcDNA3 vector alone, labeled with anti-PS-2. (b) Live cells, PS-2 transfected, labeled with anti-PS-2. (c) Live cells, PS-2 transfected, labeled with anti-PS-2 in the presence of an excess of soluble specific PS-2–KLH oligopeptide conjugate. (d) Nomarski image of c. (e) Live cells, PS-2 transfected, treated with trypsin before anti-PS-2 labeling. (f) Nomarski image of e. (g) Live cells, PS-2 transfected, labeled with anti-tubulin. (h) Nomarski image of g. (i) Fixed and permeabilized cells, PS-2 transfected, labeled with anti-PS-2. (j) Fixed and permeabilized cells, PS-2 transfected, labeled with anti-tubulin.