Skip to main content
. 2008 Feb 11;9(4):446–457. doi: 10.1111/j.1600-0854.2008.00714.x

Figure 3. Slp2-a is readily degraded and stabilized by association with Rab27a.

Figure 3

A) Western blots of mouse CTL lysates from heterozygous or homozygous ashen mice prepared and run on SDS–PAGE immediately (fresh lysate) or stored for 6 weeks at −20°C before analysis. The nitrocellulose filter was probed with anti-Slp2-a antibody, washed and reprobed with anti-Rab27a and anti-actin antibodies. WT, wild type. B) Western blots of human CTL lysates derived from healthy donors (lane 1) and patients lacking Munc13-4 (lane 2) or Rab27a (lane 3) probed with antibodies to Slp1 SHD, Slp2-a SHD or actin. Relative migration of molecular weight markers (in kiloDalton) is shown on the left.