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. 2008 Feb 11;9(4):446–457. doi: 10.1111/j.1600-0854.2008.00714.x

Figure 7. Slp2-a localizes predominantly to the plasma membrane and focuses at the immunological synapse.

Figure 7

Mouse Slp2-a tagged with pmaxGFP at either the amino terminus (panels A–C) or the carboxy terminus (panels D–I) expressed in RBL cells (panels A–I). Panels A, D and G show GFP fluorescence, and B and E show staining with anti-Slp2-a SHD antibody. Panel H shows Lgp100 staining. Panels C, F and I are merged images of A and B, D and E, and G and H, respectively. Panels J–O show N-terminally pmaxGFP-tagged mouse Slp2-a expressed in mouse CTL either unstained (panels J and L) or counterstained with anti-Lamp2 antibody ABL-93 (panel K) or anti-Slp2-a SHD antibody (panels M–O). Letter T indicates P815 target cell. All panels to the same scale. Scale bar: 10 μm.