Figure 8.

Monocyte and macrophage phenotypes in the hearts of IL-13 KO mice at day 21 of EAM. A: Representative gating of intracardiac monocyte and macrophage populations. Infiltrating CD45⁺ leukocytes are subsetted by bivariate analysis of F4/80 and CD11b expression, revealing distinct populations: UL, CD11b^hiF4/80⁻ monocytes; UR, CD11b^hiF4/80^int macrophages; LR, F4/80^hiCD11b^int macrophages. CD204 and CD206 expression are then determined, based on gates set from total CD45⁺ cells. B: CD204⁺CD206⁺ double-positive expression on CD11b^hiF4/80⁻ monocytes, as a proportion of CD45⁺ cells. C: CD204⁺ or CD206⁺ expression, as a proportion of total F4/80⁺ macrophages. CD204⁻CD206⁻ double-negative CD11b^hiF4/80⁻ monocytes (D) and F4/80⁺ macrophages (E), as a proportion of total CD45⁺ cells. Individual IL-13 KO (filled) and WT (open) animals are shown. Horizontal bars indicate the mean of each group ± the SD. Statistics are by two-tailed Student’s t-test.