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. 2008 May;172(5):1303–1311. doi: 10.2353/ajpath.2008.071139

Figure 1.

Figure 1

Immunocytochemical identification of trophoblast cells in the vCTB cell preparations used in the experiments. A: Cartoon showing locations of vCTB and syncytiotrophoblast (sTB) cells and MCs in human term placental villus. B: Identification of cytokeratin-7-positive trophoblast cells. The percentage of trophoblast cells in the vCTB cell preparations was established by centrifuging the cells onto glass slides using a Shandon Cytospin (Shandon, Pittsburgh, PA) and immunostaining for cytokeratin-7 to reveal trophoblast cells. The cells were counterstained with Mayer’s hematoxylin. The inset shows failure of binding of the isotype control mAb. Original magnifications, ×200.