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. 2008 May;172(5):1391–1402. doi: 10.2353/ajpath.2008.071004

Figure 4.

Figure 4

TGF-β1 inhibits E-cadherin and induces Slug and Snail expression in keratinocytes. A: The immunofluorescence demonstrates a diminished expression of E-cadherin (green) at the cell membrane after incubation with TGF-β1 during 48 hours. The nuclei were stained with DAPI (blue). Western blot (B) and RT-PCR (C) confirm the down-regulation of E-cadherin and show the increased expression of Slug and, at a lower level, of Snail transcription factors after TGF-β1 treatment. Results are representative of three independent experiments performed in duplicates. The mean ± SD is shown. Asterisks indicate statistically significant differences (*P < 0.05; **P < 0.01; ***P < 0.001). Original magnifications, ×630.