Figure 2.

The mouse MT-I/II locus, deletion constructs and their activity in cultured cells as assessed by β-galactosidase activity. (A) The boundary of the 10-kb 5′ flank is indicated by the EcoRI sites (E) whereas the 3′ flank is bounded by a EcoRI and XbaI (X) site. The various deletions of the 5′ region were obtained by restriction digests of the sites indicated (S, SpeI; Bg, BglII; Nh, NheI; B, BamHI; Sm, SmaI). All were tested, but only the results for the largest deletion (5′a) are shown. The construct 5′3′ΔGRE is the same as 5′3′ except that the region defined in 5′e is deleted. (B) Transcriptional response of the MRE–βGeo fusion constructs in BHK cells in response to induction with 100 μM ZnSO₄, 100 nM DEX, or both in comparison to untreated cells expressing the tested construct. All results are derived from at least three separate experiments and are expressed as the mean ± SEM.