Figure 5.

Gel mobility shift analysis of the DNA fragments tested in Fig. 4. The ability of the DNA-binding domain of the GR to retard the mobility of wild type (WT) and mutants lacking either one (D3 or D4) or both (D5) putative GREs was tested and compared with a sequence known to contain a functional GRE (hMTIIa). The specificity of the interaction was verified by the lack of interaction between GR and a fragment containing the mMTI cDNA and also by the ability to block the shift with excess unlabeled probe as indicated by + above the lanes.