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. Author manuscript; available in PMC: 2009 Mar 1.
Published in final edited form as: Insect Biochem Mol Biol. 2007 Dec 4;38(3):296–306. doi: 10.1016/j.ibmb.2007.11.011

Table 2.

Expression rates (%) and transient peak currents (Imax) of wild type and specifically mutated Vssc1 co-expressed with Vsscβ in Xenopus oocytes

AA residuea
 Nb Vssc1 variant % Expressionc Imax ± SD (n)d
827 929 932
M T L 84 Vssc1WT 53.57 1.51 ± 0.85 (13)
I T L 46 Vssc1MI 56.52 1.46 ± 1.37 (16)
M I L 58 Vssc1TI 27.59e 0.59 ± 0.17 (10)f
M T F 52 Vssc1LF 32.73e 2.32 ± 1.92 (11)
I I L 36 Vssc1MITI 38.89 2.33 ± 1.35 (13)
I T F 50 Vssc1MILF 58.00 1.46 ± 0.98 (15)
M I F 50 Vssc1TILF 26.00e 0.62 ± 0.16 (7)f
I I F 98 Vssc1MITILF 47.96 0.84 ± 0.43 (10)
a

Vssc1 variants are identified by the amino acid residues at positions 827, 929 and 932. The MTL (M827/T929/L932) variant is wildtype Vssc1 (Vssc1WT).

b

N, total number of oocytes co-injected with Vssc1 and Vsscβ cRNAs.

c

% Expression = (Oocytes producing TTX-sensitive sodium currents/total oocytes co-injected with the same amount of Vssc1 and Vsscβ cRNAs) × 100.

d

Number of eggs used to measure transient peak current.

e

Indicates percent expression of the specifically mutated Vssc1/Vsscβ variant is significantly less than that of Vssc1WT/Vsscβ (t-test, P < 0.05).

f

Amplitude of transient peak current significantly reduced compared to that of Vssc1WT/Vsscβ (t-test, P < 0.05).