Figure 5. Docking and fusion are two independent steps in the maturation of the autophagosome.

A) Low LC3/lgp120 colocalization in cell treated with bafilomycin for 24 h. B) Enlargement of area in (A); autophagosome docked onto lysosome (arrowheads) with common movement, but no fusion (see separate intensities and Figure 4). Scale bars represent 10 μm. These data are representative of five independent experiments.