Figure 8. Dynein knockdown redistributes autophagosomes towards the periphery and decreases autophagosome–lysosome fusion.

A) Mostly perinuclear localization of autophagosomes/autophagolysosomes in control cells. B) Redistribution of autophagosomes/autophagolysosomes towards the periphery with a concomitant reduction in autophagosome–lysosome fusion (see magnification). C) Quantification of reduction in colocalization of LC3 with lgp120 [three experiments with 20 cells each, p < 0.0001 (odds ratio)]. D) Quantification of colocalization of cells transfected with control (Ctrl) or DHC siRNA, mCherry–LC3 and lgp120–GFP and stained for CIMPR (20 cells each, bars represent means with standard deviations, p (LC3+/CIMPR+/lgp120+) = 4 × 10⁻⁶, p (LC3+/CIMPR−/lgp120+) = 3 × 10⁻⁵). Scale bars represent 10 μm.