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. 2008 Mar;20(3):720–738. doi: 10.1105/tpc.107.052043

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Copyright © 2008, American Society of Plant Biologists

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Figure 12. — DCB-Treated P. infestans Is Nonpathogenic.

(A) and (B) Scanning electron micrograph of wild-type cysts that have produced short germ tubes and appressoria and have penetrated a potato leaflet, 16 hpi.

(C) and (D) Cysts treated with DCB and inoculated in the presence of DCB have produced long germ tubes and appresorium-like structures and are unable to penetrate the potato leaflet, 16 hpi.

(E) Zoospores produced in the presence of DCB are completely nonpathogenic, while control zoospores produce large water-soaked necrotic lesions. All observations were performed 6 d after inoculation. (i) and (iii) Zoospores treated with 40 μM DCB, in 1.25% DMSO, and inoculated in the presence of 40 μM DCB. (ii) and (iv) Control zoospores treated with 1.25% DMSO control and inoculated in the presence of 1.25% DMSO. (v) Zoospores produced in the presence of 40 μM DCB and washed and inoculated in water are able to recover their pathogenicity. (vi) Leaflets inoculated with 40 μM DCB only show no symptoms.