Table 2.

Oligonucleotide Primer Pairs and Amplicon Sizes Used in Template Preparation for dsRNA Synthesis and Real-Time RT-PCR

Gene	Primer Name	Primer Sequence (5′–3′)	Application	Amplicon Size (bp)
Pi CesA1	CesA1T7F	GTAATACGACTCACTATAGGGCGTGCCGAAGCTTATCGT	dsRNA synthesis	240
	CesA1T7R	GTAATACGACTCACTATAGGGACAGCGCCAGAGAACACC	dsRNA synthesis
	CesA1TaqF	CTCCATTGCTGTGGGTCTCAT	Real-time RT-PCR	90
	CesA1TaqR	GGCTCAACCTAGATACACCTCCTCTA	Real-time RT-PCR
Pi CesA2	CesA2T7F	GTAATACGACTCACTATAGGGCTACCGCAGAGCTACCGAAC	dsRNA synthesis	195
	CesA2T7R	GTAATACGACTCACTATAGGGATTCCTGGATGCTCATACGC	dsRNA synthesis
	CesA2TaqF	TGTCGGCGCTGAGCAA	Real-time RT-PCR	77
	CesA2TaqR	GAACGCGTAGGCGAACCA	Real-time RT-PCR
Pi CesA3	CesA3T7F	GTAATACGACTCACTATAGGGAGATGCCTACACGGGTAACG	dsRNA synthesis	206
	CesA3T7R	GTAATACGACTCACTATAGGGGGTCGACTTCGCTCTCATTC	dsRNA synthesis
	CesA3TaqF	TTCACGCTGCTCTTCAGTCAA	Real-time RT-PCR	80
	CesA3TaqR	ACGTAGTTCCACGGGTTCGT	Real-time RT-PCR
Pi CesA4	CesA4T7F	GTAATACGACTCACTATAGGGGAGTCTGTGGCTGCTGCTCT	dsRNA synthesis	189
	CesA4T7R	GTAATACGACTCACTATAGGGGATCGGGTACACCGTCAAGT	dsRNA synthesis
	CesA4TaqF	TGGCGCTGGTGCCTAAG	Real-time RT-PCR	82
	CesA4TaqR	CGCAGCACGTCATCGTTATC	Real-time RT-PCR
GFP	GFPT7F	GTAATACGACTCACTATAGGGGCAGATTGCGTGGACAGGT	dsRNA synthesis	199
	GFPT7R	GTAATACGACTCACTATAGGGCTGGAGTACAACTACAACT	dsRNA synthesis
ActA	ActAF2	CATCAAGGAGAAGCTGACGTACA	Real-time RT-PCR	69
	ActAR2	GACGACTCGGCGGCAG	Real-time RT-PCR