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pH Optima of the Two Mature IDI Proteins from Arabidopsis.
Activity is based on the conversion of [1-14C]IPP to its acid-labile isomer, DMAPP, and scintillation counting of an ether extract of the reaction following acid hydrolysis. Error bars indicate sd of four replicates at each pH. Linear conditions were established previously by assaying a range of enzyme concentrations and incubation times. IDI1 and IDI2 are represented by open and closed circles, respectively.
