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. 2008 Mar;20(3):677–696. doi: 10.1105/tpc.107.053926

Figure 6.

Figure 6.

RT-PCR Analysis of Wild-Type and idi Mutant Lines.

Total RNA was converted to cDNA using a poly(dT) primer and then used in a PCR either with primers that amplify the full-length transcript of IDI1 (columns A to C) or IDI2 (columns D and E) (top panel) or a normalizer gene (RP2LS; bottom panel) to confirm the conversion of RNA into cDNA template. cDNA templates are as follows: wild type (A), idi1-1 (B), idi1-2 (C), wild type (D), and idi2-1 (E). IDI1 was amplified with primers flanking the start and stop codons and is predicted to produce a product of 930 bp, whereas IDI2 was amplified with primers that included a small portion of the 3′ UTR and is thus predicted to amplify a slightly larger product of 988 bp.