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. Author manuscript; available in PMC: 2008 Dec 1.
Published in final edited form as: Invest Ophthalmol Vis Sci. 2007 Dec;48(12):5733–5741. doi: 10.1167/iovs.07-0097

Figure 3.

Figure 3

Wnt signaling was activated during retinal degeneration. (A) The rd1/Tcf-LacZ mice (rd1, right) were compared with nondegenerating +/+-Tcf-LacZ littermate controls (wt, left). Wnt signaling (X-gal staining region (blue) is indicated by square brackets) in the INL layer was wider in the rd1 mice, indicating that an additional cell type(s) acquired Wnt signaling during degeneration. Retinas from P19 mice are shown. (B–E) β-galactosidase (β-gal, green, B) in the rd1/Tcf-LacZ retinas colocalized (arrows) with the Müller marker glutamine synthetase (GS; red, C). Choroidal vessels (B) are nonspecifically stained. (D, blue) DAPI-stained nuclei. (E) Merged image; original magnification, ×40. Retina from a P14 mouse is shown. (F) Higher magnification from an adjacent region of the tissue; original magnification, ×63. (G) Incubation with secondary antibodies only, at the same or greater exposure time, did not show any staining. (H, I) Region of the ONL of a P14 rd1/Tcf-LacZ retina showing colocalization of β-gal (green, H) and the microglia marker protein IBA-1 (red, I). Original magnification, ×40.