Table 1.
Difference in the transcriptional pattern of quiescent compared to stimulated CD8 T cells
| Class Comparison | # of genes differentially expressed at Student t test or F test p2-value < 0.001 | Permutation Test (p-value) Multivariate |
| Ex vivovs CD8 in culture vs IL-2 vs IL-2+Flu | 6,692 (F test, n = 24) | 0 |
| Ex vivovs combined IL-2 and IL-2+Flu | 5,527 (t test, n = 18) | 0 |
| Ex vivovs IL-2 vs IL-2+Flu | 4, 702 (F test, n = 18) | 0 |
| Ex vivovs IL-2 | 4,893 (t test, n = 12) | 0 |
| Ex vivovs IL-2+Flu | 5, 859 (t test, n = 12) | 0 |
| IL-2 vs IL-2+Flu | 1,727 (t test), n = 12 | 0 |
Ex vivo refers to the transcriptional analysis of freshly isolated and processed CD8 positive T cells; CD8 T cells were also sorted by high speed FACS sorter from PBMC from HLA-A*0201 expressing normal donors that had been cultured for 7 days in the presence of 300 IU IL-2 following stimulation with the Flu M1:58-66 peptide according to their level of CFSE and Flu/tHLA staining. CD8 expressing, CSFEhigh, Flu/tHLA negative T cells (non Flu specific) are referred to as IL-2 while CD8 expressing, CSFElow, Flu/tHLA positive T cells are referred to as IL-2+Flu