Table 2.
Candidate transcripts upregulated in hybrids compared to X. laevis.
| ProbeID | GenBankID | Target Gene | GeneSymbol | Description/Molecular Function | Mean Laevis | SD Laevis | Mean Hybrid | SD Hybrid | L-H | adj.P.Val |
|---|---|---|---|---|---|---|---|---|---|---|
| Xl.4605.1.A1_at | BG552470 | EST | MGC83384 | 3.41 | 0.39 | 7.63 | 0.44 | -4.22 | 0.0041 | |
| Xl.19075.1.A1_at | BI675584 | EST | Weakly similar to HES2 (Hairy and enhancer of split 2) (H. sapiens) | 5.53 | 1.29 | 9.67 | 0.27 | -4.14 | 0.0205 | |
| Xl.24502.1.S1_at | BJ098608 | EST | Weakly similar to forkhead box P2; (H. sapiens) | 4.56 | 0.70 | 8.65 | 0.00 | -4.09 | 0.0060 | |
| Xl.23573.1.S1_at | BC041496.1 | thymine-DNA glycosylase | TDG | Hydrolase activity, acting on glycosyl bonds | 5.55 | 1.16 | 9.22 | 0.15 | -3.67 | 0.0210 |
| Xl.16239.1.A1_at | BJ039322 | EST | 5.39 | 1.36 | 8.66 | 0.46 | -3.27 | 0.0405 | ||
| Xl.8630.1.S1_s_at | BC045272.1 | MGC53990 | MGC53990 | Similar to serum-inducible kinase; protein serine/threonine kinase act. | 4.74 | 0.36 | 8.01 | 0.75 | -3.26 | 0.0062 |
| Xl.21956.1.S1_at | BC042271.1 | MGC53461 | MGC53461 | 8.39 | 0.22 | 11.57 | 0.00 | -3.18 | 0.0041 | |
| Xl.24699.1.S1_at | CB984321 | EST | MGC79012 | Highly similar to alpha cardiac actin (H. sapiens) | 3.65 | 0.36 | 6.51 | 1.79 | -2.85 | 0.0327 |
| Xl.10600.1.S1_at | BE491023 | EST | 4.88 | 0.94 | 7.68 | 0.25 | -2.80 | 0.0256 | ||
| Xl.11324.1.A1_at | BG552091 | EST | 3.44 | 0.46 | 6.23 | 0.11 | -2.79 | 0.0064 | ||
| Xl.13010.1.A1_at | BJ099673 | EST | 5.43 | 0.46 | 8.22 | 1.89 | -2.79 | 0.0417 | ||
| Xl.14915.1.A1_at | BM180884 | EST | 5.11 | 0.30 | 7.72 | 0.35 | -2.61 | 0.0057 | ||
| Xl.13666.1.A1_x_at | BJ091634 | EST | 7.61 | 0.80 | 10.17 | 0.37 | -2.57 | 0.0230 | ||
| Xl.24516.1.S1_at | CB560511 | EST | LOC495356 | Weakly similar to Apolipoprotein E precursor (H. sapiens); lipid binding | 6.41 | 0.60 | 8.93 | 1.00 | -2.52 | 0.0244 |
| Xl.13831.3.S1_at | BJ075928 | EST | 5.64 | 0.45 | 8.05 | 0.17 | -2.41 | 0.0094 | ||
| Xl.24058.1.S1_at | BI940804 | EST | MGC82121 | Highly similar to histone H2A.FZ variant, isoform 1(H. sapiens) | 6.99 | 0.28 | 9.40 | 0.09 | -2.41 | 0.0057 |
| Xl.16509.1.A1_at | BJ084267 | EST | 4.04 | 0.35 | 6.44 | 1.00 | -2.40 | 0.0185 | ||
| Xl.433.2.S1_at | BC044959.1 | neurotrophin receptor B | trkb-b | Protein amino acid phosphorylation | 3.68 | 0.40 | 6.01 | 0.00 | -2.33 | 0.0076 |
| Xl.19610.1.A1_at | BJ084191 | EST | Similar to Angiopoietin-1 receptor precursor (mTIE2) | 4.48 | 0.05 | 6.81 | 0.36 | -2.33 | 0.0043 | |
| Xl.75.1.S1_at | D78003.1 | c4 | c4 | Endopeptidase inhibitor activity i fourth component of complement | 4.09 | 0.47 | 6.41 | 0.00 | -2.32 | 0.0101 |
| Xl.882.1.S1_at | U07179.1 | Ldehydrogenase A | ldha | Oxidoreductase activity | 5.29 | 0.94 | 7.60 | 0.06 | -2.31 | 0.0387 |
| Xl.17327.1.A1_at | BI448285 | EST | MGC68503 | 4.77 | 0.49 | 7.07 | 0.19 | -2.29 | 0.0117 | |
| Xl.747.1.S1_at | AF170341.1 | galectin-1 | MGC64502 | Sugar binding | 5.51 | 0.21 | 7.80 | 0.55 | -2.29 | 0.0075 |
| Xl.545.1.S1_at | AF170344.1 | metastasis associated 1 | mta2 | Transcription factor activity, regulation of transcription | 7.85 | 0.51 | 10.14 | 0.13 | -2.29 | 0.0124 |
| Xl.23647.1.S1_at | BC047974.1 | cell death 2 | pdcd2 | Apoptosis | 4.11 | 0.36 | 6.34 | 0.40 | -2.23 | 0.0096 |
| Xl.19047.1.A1_at | BI478140 | Coatomerprotein | copa | ER to Golgi vesicle-mediated transport i | 8.05 | 0.35 | 10.28 | 0.01 | -2.23 | 0.0071 |
| Xl.9113.1.A1_at | BG346438 | chimerin | chn1 | Signal transduction | 6.10 | 0.76 | 8.32 | 0.12 | -2.22 | 0.0276 |
| Xl.16847.1.A1_at | BJ052360 | EST | 4.22 | 0.74 | 6.44 | 0.36 | -2.22 | 0.0279 | ||
| Xl.13666.1.A1_at | BJ091634 | EST | 6.81 | 0.77 | 8.97 | 0.54 | -2.16 | 0.0350 | ||
| Xl.721.1.S1_at | L09728.1 | transcription factor DLL4 | Dlx2 | Regulation of transcription, DNA-dependent | 7.89 | 0.58 | 10.04 | 0.35 | -2.15 | 0.0199 |
Top 30 candidate transcripts upregulated in hybrids and differentially expressed between females of X. laevis and hybrids. Expression values are in log2 scale; SD = standard deviation of expression values. P values are adjusted according to FDR moderated t-tests.