Table 4.
Candidate transcripts upregulated in hybrids compared to X. muelleri.
| ProbeID | GenBank ID | Target Gene | GeneSymbol | Description/Molecular Function | Mean Muell. | SD Muell. | Mean Hybrid | SD Hybrid | M-H | P value |
|---|---|---|---|---|---|---|---|---|---|---|
| Xl.4276.1.S1_at | X53745.1 | Cyclin A1 | LOC397885 | Regulation of progression through cell cycle | 5.12 | 0.58 | 12.76 | 0.10 | -7.64 | 0.0001 |
| Xl.8319.1.S1_at | BJ098891 | Herz03 | Herz03 | 4.84 | 0.22 | 11.65 | 0.03 | -6.80 | 0.0001 | |
| Xl.21809.1.S1_at | BC041555.1 | MGC53900 | MGC53900 | Similar to calcium modulating ligand | 3.00 | 0.31 | 9.78 | 0.23 | -6.79 | 0.0001 |
| Xl.17345.1.A1_at | BJ053357 | EST | MGC115708 | Weakly similar to MIC2_HUMAN T-cell surface glycoprotein E2 precursor (H. sapiens) | 3.33 | 0.21 | 10.03 | 0.99 | -6.70 | 0.0002 |
| Xl.4744.1.S1_at | BE491637 | LOC495025 | Moderately similar to ubiquitin thiolesterase (H. sapiens); ubiquitin-dependent protein catabolism | 4.78 | 0.60 | 11.43 | 0.16 | -6.65 | 0.0002 | |
| Xl.6585.1.S1_at | BJ080015 | Similar to HIV-1 rev binding protein 2 | HRB2 | RNA binding | 3.22 | 0.20 | 9.75 | 0.14 | -6.53 | 0.0001 |
| Xl.21357.2.S1_at | BJ045324 | Claudin7L1 | MGC53400 | Xenopus laevis cldn7L1 mRNA for Claudin7L1, structural molecule activity | 3.90 | 0.27 | 10.42 | 0.03 | -6.51 | 0.0001 |
| Xl.14775.1.A1_at | BM179359 | EST | Weakly similar to POL2_MOUSE Retrovirus-related POL polyprotein (M. musculus) | 3.81 | 0.18 | 10.23 | 0.22 | -6.43 | 0.0001 | |
| Xl.3668.1.S1_at | AF450296.1 | XLCL2 | LOC397879 | Xenopus laevis F-box protein (PXP17), meiosis | 4.46 | 0.60 | 10.82 | 0.06 | -6.36 | 0.0002 |
| Xl.3401.2.A2_at | BG016692 | EST | LOC446970 | Similar to axotrophin; likely ortholog of mouse axotrophin (H. sapiens), protein binding | 3.72 | 0.63 | 9.95 | 0.14 | -6.24 | 0.0002 |
| Xl.1018.1.A1_at | U44950.1 | Vitelline envelope glycoprotein | lzpb-a | Xenopus laevis vitelline envelope 37 k glycoprotein xlZPB | 4.10 | 0.53 | 10.31 | 0.29 | -6.22 | 0.0002 |
| Xl.3862.2.S1_x_at | CD361360 | Translation factor sui1 | gc20 | Translation initiation factor activity | 3.29 | 0.41 | 9.45 | 0.17 | -6.16 | 0.0001 |
| Xl.7151.1.S1_at | BJ089477 | EST | MGC68561 | Moderately similar to hypothetical protein FLJ10738 (H. sapiens); 3'-5' exonuclease activity | 4.69 | 0.31 | 10.84 | 0.21 | -6.15 | 0.0001 |
| Xl.3536.2.S1_x_at | BF615663 | EST | LOC495200 | Highly similar to transcription factor BTF3a – (H. sapiens) | 4.19 | 0.26 | 10.32 | 1.21 | -6.13 | 0.0004 |
| Xl.23448.1.S1_at | BC041216.1 | SWI/SNF | smarce1 | Similar to SWISNF, actin dependent regulator of chromatin, regulation of transcription | 3.75 | 0.62 | 9.88 | 0.23 | -6.13 | 0.0002 |
| Xl.2060.1.A1_x_at | BJ055271 | EST | 3.61 | 0.40 | 9.69 | 1.64 | -6.08 | 0.0008 | ||
| Xl.576.1.S1_at | AF184090.1 | fatvg | fatvg | 4.94 | 0.56 | 11.02 | 0.03 | -6.08 | 0.0002 | |
| Xl.24785.1.S1_at | BM261081 | EST | MGC81067 | 4.41 | 0.07 | 10.41 | 0.03 | -5.99 | 0.0001 | |
| Xl.4504.1.A1_at | BJ076394 | EST | Weakly similar to ACRC protein; putative nuclear protein (H. sapiens) | 4.11 | 1.10 | 10.10 | 0.06 | -5.99 | 0.0007 | |
| Xl.7045.1.S1_a_at | BQ398421 | EST | Weakly similar to CTF1_HUMAN Cardiotrophin-1 (CT-1) (H. sapiens) | 2.67 | 0.16 | 8.64 | 0.24 | -5.97 | 0.0001 | |
| Xl.7252.1.S1_at | AY172320.1 | Germes | LOC398520 | 3.60 | 0.18 | 9.55 | 0.27 | -5.96 | 0.0001 | |
| Xl.2565.3.S1_x_at | CB561588 | Similar to alpha-Tubulin at 84B | MGC53359 | Xenopus laevis, Similar to alpha-Tubulin at 84B, microtubule-based movement | 4.44 | 0.41 | 10.39 | 0.27 | -5.95 | 0.0002 |
| Xl.7837.1.A1_at | BF232270 | EST | MGC132211 | Highly similar to hypothetical protein FLJ10900 (H. sapiens), electron transport | 2.94 | 0.43 | 8.88 | 0.02 | -5.94 | 0.0001 |
| Xl.25809.1.A1_at | BE026874 | EST | MGC80281 | Histidine catabolism | 4.26 | 0.49 | 10.18 | 0.36 | -5.92 | 0.0002 |
| Xl.6605.1.A1_at | AW632842 | EST | 3.59 | 0.26 | 9.50 | 0.02 | -5.91 | 0.0001 | ||
| Xl.4170.2.A1_at | BQ398301 | LOC494857 | LOC494857 | Cell differentiation | 4.21 | 0.59 | 10.11 | 0.11 | -5.90 | 0.0002 |
| Xl.1014.1.S1_at | U46131.1 | Cdc21 protein | cdc21 | Xenopus laevis DNA replication initiator protein, DNA replication initiation, regulation of transcription | 4.14 | 0.46 | 10.00 | 0.08 | -5.86 | 0.0002 |
| Xl.2839.1.S1_at | BC041270.1 | Protein translocation complex | sec61beta | Similar to protein translocation complex beta | 5.91 | 0.17 | 11.76 | 0.08 | -5.85 | 0.0001 |
| Xl.25536.1.A1_at | BE677987 | EST | Weakly similar to hypothetical protein MGC2577 (H. sapiens) | 4.36 | 1.14 | 10.15 | 0.93 | -5.79 | 0.0012 | |
| Xl.14065.1.A1_at | AW147826 | EST | 3.61 | 0.15 | 9.33 | 0.12 | -5.72 | 0.0001 |
Top 30 candidate transcripts upregulated in hybrids and differentially expressed between females of X. muelleri and hybrids. Expression values are in log2 scale; SD = standard deviation of expression values. P values are adjusted according to FDR moderated t-tests.