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Effect of over expression of candidate genes on the CFC frequency. A total of 80 CD34−KSL HSCs (10 cells/well) were cocultured with PA6 cells, PA6 S-2 cells, or PA6 S-2 cells expressing the indicated genes for 10 days and then subjected to the CFC assay. Individual colonies were scored according to their morphology. Data represent the average of two independent experiments
