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. 2008 May 7;2(5):e231. doi: 10.1371/journal.pntd.0000231

Figure 1. NF-κB activity in response to M. leprae is diminished in the 1805T variant.

Figure 1

HEK293 cells were transfected with an NF-κB luciferase reporter, a Renilla luciferase construct to control for transfection efficiency (pRL-TK), and CD14. Additional transfectants varied by condition and included: empty plasmid vector (EV, clear), TLR2 alone (T2, gray), or TLR2 with one of two TLR1 constructs, 1805T (black) or 1805G (stippled). Luciferase activity represents basal (media stimulation) or stimulated activity of transfected cells. Mean values (+/−standard deviation) are depicted for two representative experiments, each performed in triplicate. RLU, relative luciferase units; P3 300, PAM3 at a dose of 300 ng/ml; M2 100, Malp-2, at 100 ng/ml; ML, whole, irradiated ML at 5, 50, or 250 µg/ml; MLcw, M. leprae cell wall at a dose of 1 or 10 µg/ml. * = P≤0.01, by Student's t-test when comparing the 1805T and 1805G variants (both with T2); # = P≤0.01, by Student's t-test when comparing T2+1805T and T2; ˆ = P≤0.01, by Student's t-test when comparing T2+1805G and T2.