Table 3.

B. subtilis alanine substituted AT proteins binding to TRAP

B.subtilis AT	Residue location on AT3	Kd (µM)‡
W.T.	wild type	3.1 ± 0.1
V02A	top	n.b.*
D06A	top	n.b.*
E22A	end of the arm	5.9 ± 1.0
N52A	bottom	2.2 ± 0.5

^¶Location of AT residues tested for alanine substitution (See Figure 5A).

^‡Each value is the average ± the standard deviation of at least two independent binding measurements, each done in triplicate.

^*n.b., no binding detected with AT₃ up to 60 µM.