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High-level endogenous globin gene expression is observed only with wild-type p45NF-E2 expression in erythroid cells. RNase protection assays for α-globin, β-major mRNAs from CB3 cells, CB3 cells stably transfected with various p45 NF-E2 expressing constructs, and MEL cells. Actin levels were measured in parallel in all samples.
