Table 1.

Globin gene expression in transfected CB3 cell lines

	Fold activation
	β-globin	α-globin
Empty vector	1	1
NF-E2 wild type	5.3 ± 0.4*	4.1 ± 0.2*
NF-E2Δ80	0.9 ± 0.3	0.85 ± 0.2

Individual CB3 clones expressing wild-type or mutant NF-E2 cDNAs were assayed for endogenous globin gene expression using specific α- and β-globin specific probes. Variability in the amount of RNA loaded per assay was corrected internally by analyzing the expression of β-actin (see Fig. 5). Comparison of activation of the globin genes were corrected with respect to untransfected CB3 cells. The results shown are the average of at least three independent experiments. 

^*Significantly different from CB3 cells alone (P < 0.01).