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. 2007 Jan 17;63(Pt 2):78–83. doi: 10.1107/S1744309106055084

Figure 2.

Figure 2

(a) Elution profile from the cation-exchange purification step for the H6 DENV 3 RdRp protein. The linear gradient ranges from 0.15 M NaCl (corresponding to the inclusion of 10% buffer B) to 1.5 M NaCl (100% buffer B). Two major fractions F1 and F2 were then subjected to gel filtration and initial crystallization trials (see text). (b) SDS–PAGE analysis. Lane 1, molecular-weight markers (kDa). Lanes 2 and 5, protein DENV 3 RdRp from the minor fraction that elutes first. Lanes 3 and 4, fractions F1 and F2, respectively, both of which contain DENV 3 RdRp.