Table 2. Selected crystal data and diffraction data statistics.
Data were collected on the 5-ID beamline at APS and were processed using the program AUTOMAR (Bartels & Klein, 2003 ▶). Values in parentheses are for the outer resolution shell.
| Complex 1 | Complex 2 | |
|---|---|---|
| Protein | E. coli RNase HI | E. coli RNase HI |
| RNA duplex | CUG GCU CGC (Y5) | GCA CUU GAC C (Y6) |
| GCG AGC CAG (R5) | GGU CAA GUG C (R6) | |
| CCD detector | MAR Mosaic 225 | MAR Mosaic 225 |
| Wavelength (Å) | 1.000 | 1.000 |
| Space group | P21 | P212121 |
| Unit-cell parameters | ||
| a (Å) | 42.13 | 68.10 |
| b (Å) | 61.42 | 78.19 |
| c (Å) | 121.28 | 106.63 |
| β (°) | 93.16 | — |
| Resolution range (Å) | 30.0–3.50 (3.62–3.50) | 30.0–3.99 (4.14–3.99) |
| Observations | 36900 | 25887 |
| Unique reflections | 7476 (739) | 5099 (490) |
| Completeness (%) | 91.3 (94.6) | 98.6 (95.4) |
| Rmerge | 0.102 (0.359) | 0.061 (0.202) |