Figure 5.

(a) The effect of the concentration of lecithin on percentage haemolysis. The reaction medium contains 1.5%(v/v) erythrocytes, 10 µM Ca²⁺ and 50 µg CLBPah. The reaction was incubated at 310 K for 15 min. (b) The effect of Ca²⁺ on the percentage of haemolysis. The effect of Ca²⁺ was determined by using four Ca²⁺ concentrations. The medium consisted of 2.25%(v/v) erythrocytes, 0.025% lecithin and 50 µg CLBPah. The time and temperature of reaction were 15 min and 310 K, respectively. (c) The effect of erythrocytes on the percentage of haemolysis. Three concentrations of erythrocytes were examined. The medium contains 0.025% lecithin, 10 µM Ca²⁺ and 50 µg CLBPah. The temperature and time of reaction were 310 K and 15 min, respectively. (d) The effect of concentration of CLBPah on the percentage of haemolysis. The concentration of erythrocytes was 2.25%(v/v) and the concentration of lecithin was 0.025%. The concentration of Ca²⁺ was 10 µM.