Figure 1.

Ability of sequential R5 isolates to bind and utilize DC-SIGN for trans-infection. (A) DC-SIGN binding ability assessed as percentage specifically DC-SIGN associated p24 antigen. Efficiency of DC-SIGN mediated trans-infections analysed as ratios of p24 antigen release in DC-SIGN mediated PBMC (B) or T-cell line C6 (C) infections over p24 antigen release in directly infected PBMC and C6 cultures respectively. Presented data are average from results obtained in two or three assays performed. *, p < 0.05