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. 2008 May 9;4(5):e1000059. doi: 10.1371/journal.ppat.1000059

Figure 1. Conserved amino acid residues in Vpx C-terminal region mediate the association with DDA1-DDB1-VprBP complex.

Figure 1

(A) hfa-tagged wild type (lane 2) or mutant (lanes 3–6) Vpx proteins were transiently expressed in HEK 293T cells and precipitated from detergent extracts with FLAG-M2 affinity resin. DDB1, VprBP, DDA1 and hfa-Vpx were detected in immune complexes (left panel) and cell extracts (right panel) by immunoblotting and visualized by enhanced chemiluminescence. (B) Amino acid sequences of the C-terminal regions of SIVmac 239 Vpx, SIVmac 239 Vpr, and HIV-1 NL43 Vpr are aligned and amino acid substitutions for the conserved residues in Vpx are indicated. Numbers indicate the positions of the first amino acid residue shown in each protein sequence. Dots identify amino acid identities and letters specify amino acids in the single-letter code.