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. 2008 May 7;3(5):e2127. doi: 10.1371/journal.pone.0002127

Figure 1. cAMP reporter, enhanced by a mutation in YFP.

Figure 1

A, B. CHO cells, stably expressing rtTA, were cotransfected with RII-CFP and either the mutated (F46L, top) or original (lower) C-YFP. Note similar CFP fluorescence (left) but enhanced YFP fluorescence (right) for the F46L mutant. C. Transfected cells from A. and B., functionally imaged for FRET and stimulated with10 µM Fsk+100 µM IBMX to elevate cAMP levels. The F46L mutant cAMP reporter (•) yields a larger peak FRET signal (F470/F535) than the original reporter (○) (mean±s.e.m., n = 18 cells).